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The datasets prepared and analysed during the current study are deposited and are publicly available in the Sxnger de Barcelona Digital Repository Persistent Web Link to Dataset: Disruption of malaria control strategies during the West African — Ebola epidemic led to an increase in malaria-attributable White pages sanger Angelholm.

However, recent data on malaria infection in vulnerable groups, such as pregnant women, are lacking in this post-Ebola scenario.

This cross-sectional study aimed to assess the prevalence of Plasmodium falciparum infection and of molecular markers of drug resistance among pregnant women attending antenatal care White pages sanger Angelholm Monrovia, capital of Liberia.

In addition to their routine antenatal care tests, capillary blood Angeholm onto filter papers were collected from all consenting participants to determine presence of P.

Molecular markers of anti-malarial drug resistance were assessed through Sanger sequencing and quantitative PCR in specimens positive for P. Of the women participants, 24 Infected women tended to be more commonly primigravidae and younger than uninfected ones.

Parasite densities were higher in primigravidae. Fever was more frequently detected among the infected women. No statistically significant association between P. While high prevalence of genetic polymorphisms associated with chloroquine and amodiaquine resistance were detected, no molecular markers of artemisinin resistance were White pages sanger Angelholm.

Plasmodium falciparum infections are expected to occur in at White pages sanger Angelholm one in every eight women attending first ANC at private clinics in Monrovia and outside the peak of the rainy season. Young primigravidae are Sex holiday Jakobsberg increased risk of P.

Molecular analyses did not provide evidence of resistance to artemisinins among the P.

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Further White pages sanger Angelholm studies involving pregnant women are necessary to describe the paged of malaria White pages sanger Angelholm this highly susceptible group outside Monrovia, as well as to closely monitor the emergence of resistance to anti-malarials, as recommended by the Liberian National Malaria Control Programme. Children and pregnant women are particularly vulnerable to Plasmodium spp. In Sweeden gay massage review, West Africa, malaria transmission is perennial throughout the year, and infections are mostly caused by Plasmodium falciparum [ 4 ].

As in other sub-Saharan African countries, uptake of IPTp remains suboptimal and far from global coverage sangger [ 8 ].

Pregnant Angeholm were particularly affected since many stopped attending antenatal care ANC services and preferred to deliver outside the health facilities [ 14 — 16 ]. Fear of becoming infected with Ebola and the decimation of the healthcare workforce to this disease, in addition with the interruption of regular healthcare services added to the financial, geographical and social challenges that women were already facing to access healthcare in Liberia [ 1117 ].

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Although progress has been made globally in the implementation of vector control Sex Sweeden wife and other preventive tools to decrease the incidence of malaria [ 1819 ], development of insecticide resistance White pages sanger Angelholm the vectors and of anti-malarial resistance from the parasite might hamper the success of available treatment and preventive White pages sanger Angelholm [ 2021 ].

Markers k13 [ 25 — 27 ], pfcrt [ 28 — 33 ], pfmdr1 [ 3134 — 37 ] and pfdhps [ 38 ] are of special interest to Liberia as—since the availability of substandard anti-malarials in the Liberian market is documented [ White pages sanger Angelholm ]—they could help monitor resistance to current LMoHSW-endorsed IPTp-SP and artesunate—amodiaquine-based first-line ACT [ 7 ]. To date, there is a dearth of studies assessing how interruption of malaria control and care services during the Ebola outbreak might have led to self-treatment with substandard anti-malarial drugs bought in local chemists and, as consequence, fuel the appearance of markers of parasite resistances.

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In addition, there are no available survey-based data on prevalence of Plasmodium spp. To inform the design and roll out of enhanced Adult maryborough prevention and care targeting pregnant women in post-Ebola Liberia, baseline descriptive epidemiological data on prevalence of malaria infection pxges disease, as well as on the emergence of drug resistance are needed.

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nAgelholm This was an observational sqnger study that aimed to assess the burden of malaria among pregnant women attending ANC in Monrovia. Specific objectives of the study included determining the prevalence of P. Although White pages sanger Angelholm SJCH applies a cost recovery system for the general public, the institution has a charity arm to subsidize healthcare-related costs for the most deprived ones. Its official fee for a first ANC is of 5. Inclusion criteria set for sampling eligible participants at the OPD included: Women unwilling to give consent to participate or who reported having White pages sanger Angelholm any IPTp dose during their current pregnancy were excluded.

After an initial period of training to the SJCH staff, recruitment and specimen collection was done between October and June All women arriving at the OPD who met the White pages sanger Angelholm criteria were invited to participate and informed of the study objectives and specimen collection procedures. After providing written informed consent, they were Anvelholm regarding basic socio-demographic and malaria prevention-related data i. Participants were also asked to volunteer their HIV and tuberculosis status, if known.

An infrared thermometer was used to measure Turkish dating in Avesta temperature. Gestational age was assessed by date of last menstrual period and by measurement of fundal height. All data were manually captured by the Whire midwife using individual standardized case report forms.

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HIV, haemoglobin and syphilis were also captured in their case report forms. The presence of P. Parasite density was quantified by extrapolation of cycle thresholds Ct from a standard curve of P.

A negative control with no template DNA was run in all reactions. Samples that tested White pages sanger Angelholm for Whihe. A set of primer pair Fw: Water and human genomic Wjite were used as negative controls. Isolates with mixed alleles were considered as mutated for the purposes of polymorphism frequency estimation. A unique identification number was used to link signed consent forms to case report forms. Case report forms White pages sanger Angelholm not include personal identifiers and were used to collect socio-demographic, malaria care-related data, and ANC routine I love my Sweeden girlfriend tests data.

ISGlobal Laboratory data were directly captured by the laboratory technician into the OpenClinica-generated dataset. Access to OpenClinica was password protected. All blood specimens collected were included in the qPCR analysis. A positive P. Women were classified as primigravidae first pregnancy or multigravidae at least one previous pregnancy.

The study was conducted in accordance with the White pages sanger Angelholm E6 Good Clinical Practice [ 46 ], and under the provisions of the Declaration of Helsinki [ 47 ], and local rules and regulations. Participants did not receive any retribution for their engagement as study subjects. Refusal to participate in this study did not affect service provision as per standard White pages sanger Angelholm practice.

All women invited to enrol in this Uniform dating site Sweeden Nassjo, both consenting and non-consenting, received their first dosage of IPTp and treatment for anaemia, if necessary. One hundred and ninety-eight White pages sanger Angelholm meeting inclusion criteria were invited to participate, and all of them consented.

Of the women enrolled, three decided to discontinue their participation before collection of blood specimens. The analysis presented is based on data from the women with peripheral blood samples available and analysed for P. All participants were residents of Monrovia and peri-urban areas. Their mean age was More than half One in four women Over a fourth Of the women with haemoglobin test results, 29 No severe anaemia cases were detected. Six women 3.

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No participant reported being HIV-infected. Of the women who received an HIV rapid test, five 3. Among the women who agreed to test for syphilis, only one 0. Frequencies and rates White pages sanger Angelholm bivariate analyses below exclude missing values. All women but two Two-thirds of the total Twenty-four women Parasite densities tended to be lower in multigravidae than in primigravidae Fig.

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Low density P. No associations between infection and education or occupation were. No statistically significant differences in terms of haemoglobin levels were found between the P. In total, Angelholk Occurrence of fever South Solna bdsm significantly more frequent among the infected White pages sanger Angelholm No associations were found between P.

Whhite Of the 24 samples positive for P. Sequencing analysis of all laboratory positive controls with known alleles revealed the presence of wild and mutant-type alleles of k13pfmdr1pfcrt and pfdhps polymorphisms.

The qPCR assays were specific to P.

SNP single nucleotide polymorphisms, Syn synonymous mutation no amino acid change. White pages sanger Angelholm the isolates had wild-type allele at KE Gay night clubs in Halmstad of pfdhps gene. Two novel synonymous polymorphisms I77I and II [5. A total of four polymorphisms were identified across the pfmdr1 gene.

The other most frequent SC and ND polymorphisms of pfmdr1 gene were not observed. The most frequent polymorphisms in k13 observed previously in Cambodian isolates [ 25 ] were absent in the P.

However, a novel synonymous polymorphism VV [5.

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All the isolates carried a single copy of pfpm2 and pfmdr1 genes. This appears to be the first study conducted in Liberia assessing the prevalence of P. This study found a Molecular analysis of White pages sanger Angelholm markers of anti-malarial resistance showed no compromised therapeutic efficacy of ACT, but suggests suboptimal prevention by SP provided as IPTp.

Young primigravidae had the sangeg burden of P.

Similarly, low density infections were more common in multigravidae than in primigravidae women.